Kristin Kreuk was born in Vancouver, British Columbia, Canada, to Deanna Che, who is of Chinese ancestry, and Peter Kreuk, who is of Dutch descent. Her parents are landscape architects. She attended and graduated from Eric Hamber Secondary School. Although she did some stage work in school, she focused more on her studies. She described herself as shy and boring.

For her first professional work, she went to an open casting audition for Edgemont (2000), a teen drama series aired on CBC Television in Canada. She plays the role as "Laurel Yeung". Laurel was the last main character to be cast because the casting director was having a problem finding an actress. Fortunately, for them (and us), they found Kristin just in time.

Her career was set to take off when she landed on the highly acclaimed serial drama series for The WB, Smallville (2001). It follows the story of "Clark Kent" in his pre-Superman days in a small town of Smallville, Kansas. Kreuk played the role as "Lana Lang", Clark Kent's object of desire before he meets "Lois Lane".

Smallville (2001) proved to be a giant step for Kristin's acting career, as she landed several interviews with several magazines, including YM (Young and Modern) and Rolling Stone. She also did a commercial for Neutrogena, popular for its skin care products.