Timo Jacobs started his movie career in 2003 in the movie "3 Minuten Heroes", directed by the german director legend Klaus Lemke. Together with Lemke, four more movies with Jacobs as leading actor followed, as well as many more international and german TV and cinema-productions.

2008, Timo Jacobs acted in the movie "The Man Who Will Come" (L'Uomo Che Verra) by Giorgio Dirittis, together with actress Alba Rohrwacher. The movie was award with the Grand Prize of the Jury at the Rome International Film Festival in 2009. For his role in "Beach Boy", Timo Jacobs was awarded the New Berlin Film Award. For his leading role in "Pete the Heat!" he was awarded with the Montblanc Movieprize at the Hamburg Film Festival in 2001. In the war epic by Spike Lee "Buffalo Soldiers" he played a Sniper in the german Wehrmacht and in the hooligan drama "Gegengrade" the leading role at the side of Mario Adorf in 2011. In 2014 he was awarded with the "Bambi" in the category TV-Event of the Year for his participation in "Bornholmer Straße". For his appearance in "Tatort: Borowski und der Himmel über Kiel" by director Christian Schwochow, Jacobs was nominated for the German Actor Award 2016.

Since 2016 he is part of the main cast of the german crime series "Der Kriminalist". He just finished shooting for various episodes in german shows in 2017, among others: Wilsberg, Bad Cop, Welser.

Timo Jacobs is also producer, director, writer and leading actor of two movies. 2011 his first movie "Klappe Cowboy!" was released and awarded with the audience award at the Achtung Berlin Film Festival, the prize of the Deposhir Film festival in St. Petersburg, Russia, as well as the prize of the jury at the International Film Festival in Louisville KY.

His second movie with him as producer, director, writer and leading actor "Mann im Spagat - Pace, Cowboy, Pace" was released german cinemas in 2017.